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Iron and zinc are important minerals in humans in sub Saharan Africa, whose deficiency is known as "hidden hunger" due to the lack of recognised symptoms in the early stages. Although iron deficiency is the most prevalent, zinc is also involved in inhibition of replication of viruses, including the corona virus (COVID-19). In North Kivu and South Kivu provinces where more than 50% of common bean is produced and consumed in Democratic Republic of Congo, 36% and 47% of preschool children are anemic due to iron deficiency. This paradox is mainly due to insufficiency of iron-rich foods. The aim of this study is to characterise 59 iron and zinc biofortified varieties together with six local varieties of common bean for a potential selection programme in Butembo town in the Democratic Republic of Congo. We focused on 15 qualitative and five quantitative parameters. The qualitative parameters were helpful to distinguish the different morphotypes and for cluster analysis. In addition to the descriptive statistics, the quantitative data were used for Pearson correlation and for principal component analysis, PCA. Qualitative parameters enabled grouping of the study genotypes into 14 morphotypes according to the aspect and colour of the seed coat, the colour around the hilum and the size of seeds. Clustering grouped the 65 genotypes into 12 clusters with the most similar genotypes grouped in the same cluster. Quantitative parameters showed that the study genotypes were dissimilar (P=0.00). A positive correlation was obtained between the days to flowering and the days to maturity (P<0.05) and between the number of pods per plant and the days to flowering. A strong correlation was found between the number of pods per plant and seeds per pod (P<0.01). In contrast, a negative correlation was observed between the 100 seed weight and the number of seeds per pod. The PCA represented on two perpendicular axes showed 64.1% of the total variance of which the 42.3% is explained by the first axis and 21.8% by the second axis. Overall, the study genotypes are morphologically and quantitatively different and thus can be used in a selection programme.
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Romania ranks first in the European Union for the production of sunflower seeds, third for the production of soybeans and seventh for the production of rapeseed. The paper aims to analyse the effects produced by the COVID-19 pandemic on the evolution of the oilseed sector in Romania. Thus, the following indicators were analysed: evolution of areas under oilseeds, total oilseed production and average yields, as well as the volatility of selling prices for oilseeds. The results of the study reveal that Romania has been the largest producer of sunflower seeds in the European Union. The average yields in sunflower, soybeans and rapeseed have shown great variations in the analysed period. According to Eurostat data, it can be noticed that although Romania is the third large producer of oilseeds in the EU, the average yields continue to be low compared to those from other large EU producers. Yields are also among the most volatile in the EU. The selling prices for soybeans showed a higher increase in the year 2020 than in 2019 in Romania, compared to the increase in the average selling prices of the EU-27 (+9.89%). The selling prices for rapeseed also had a higher increase in 2020 than in 2019 in Romania, compared to the increase in the average selling prices of the EU-27 (+2.31%).
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Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly discovered enteric coronavirus, is the etiological agent that causes severe clinical diarrhea and intestinal pathological damage in piglets. In this study, Vero E6 and IPI-2I cells were pretreated with different concentrations of glycyrrhizin (GLY) for 2 hours, and then infected with different concentrations of SADSCoV, aiming to investigate the inhibitory effect of GLY on SADS-CoV. Western blot and TCID50 results revealed a significantly decreased N protein expression and viral titer, indicating that GLY can inhibit the infection of SADS-CoV. Vero E6 and IPI-2I cells were pretreated with different concentrations of GLY for 2 hours and infected with SADS-CoV. Western blot results showed that when the concentration of GLY was 0.8 mmol/L, the expression of N protein decreased significantly, indicating that GLY inhibited the invasion of the virus. At first, cells were treated with 0.4 mmol/L GLY, and cell samples were collected at 2 hours, 6 hours and 12 hours after being infected with SADS-CoV for analysis, and the expression of N protein were found to be significantly reduced at all points, indicating that GLY had a significant inhibitory effect on the replication of the virus. GLY is a competitive inhibitor of high mobility group box 1 (HMGB1), and the receptors of HMGB1 mainly include TLR4 and RAGE. Based on this fact, the mutant plasmid at the key sites of HMGB1 (C45S, C106S, C45/106S) and the siRNA of the RAGE receptor were transfected to Vero E6 cells and infected with SADS-CoV, and the cell supernatant and samples were harvested. The western blot and TCID50 results showed that the expression of N protein and the virus titer were decreased, suggesting that GLY exerts its function by affecting the binding of HMGB1/TLR4/RAGE during SADS-CoV infection. To further explore the signaling pathway through which GLY functions, Vero E6 and IPI-2I cells were inoculated with SADS-CoV, and cell samples were harvested, western blot was used to detect the changes of MAPK proteins. The results showed that the protein expression levels of p-p38, p-JNK and p-ERK were up-regulated in the early and late stages, indicating that the MAPK pathway was activated by SADS-CoV infection. Vero E6 and IPI-2I were pretreated with different concentrations of GLY and TLR4 inhibitor TAK for 2 hours and infected with SADS-CoV. Protein samples were harvested and analysed by western blot which showed a decreased p-JNK and N proteins, while other proteins showed no significant changes. These results indicated that GLY and TAK regulated the phosphorylation of JNK but did not regulate the phosphorylation of p38 and ERK. Also, Vero E6 cells were treated with HMGB1 antibody, the siRNA of HMGB1 and HMGB1 mutants plasmid, and infected with SADS-CoV. Protein samples were harvested, western blot results showed that phosphorylation of JNK decreased, indicating that HMGB1 affected JNK phosphorylation. Finally, Vero E6 and IPI-2I cells were pretreated with different concentrations of JNK inhibitor SP600125 to infect SADS-CoV, western blot, TCID50 and IFA results showed that the expression of N protein and virus titer, as well as virus replication were reduced, indicating that SP600125 inhibited virus replication. In conclusion, our results revealed that GLY can inhibit in vitro replication of SADS- CoV, mainly through the HMGB1/TLR4/JNK signaling pathway. The discovery of this pathway provides theoretical support for the research of novel anti-SADS-CoV drugs.
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The novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in Wuhan, China, in December 2019 and quickly spread across the worldwide. It becomes a global pandemic and risk to the healthcare system of almost every nation around the world. In this study thirty natural compounds of 19 Indian herbal plants were used to analyze their binding with eight proteins associated with COVID -19. Based on the molecular docking as well as ADMET analysis, isovitexin, glycyrrhizin, sitosterol, and piperine were identified as potential herbal medicine candidates. On comparing the binding affinity with Ivermectin, we have found that the inhibition potentials of the Trigonella foenum-graecum (fenugreek), Glycyrrhiza glabra (licorice), Tinospora cordifolia (giloy) and Piper nigrum (black pepper) are very promising with no side-effects.
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The study is a review of natural raw materials that can prevent infection and help treat viral infections, including those that cause COVID-19. The condition of not getting infected with pathogens that cause infections of the upper and lower respiratory tract is high the body resistance. An important element that influences the proper immunity of the body is the diet. The functioning of the immune system is improved by bee products, and plant materials: purple coneflower herb, flower and root, licorice root, aloe gel and Baikal skullcap root, as well as black cumin seed oil, chaga mushroom, lemon balm leaves and chamomile flowers. Strengthening immunity is conducive to maintaining a good mood and reducing stress. The antiviral activity has been confirmed for many plant materials, especially those containing essential oils. Natural products can be used for prevention and treatment. The country that copes best with the coronavirus epidemic is China, thanks to a combination of academic and natural medicine. The study quotes an excerpt from the "Handbook of COVID-19 Prevention and Treatment", prepared by Chinese doctors, with particular attention to the recipes used by them.
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Unique plants and their properties, once considered synonymous to medicine, remain a potent source for new compounds in modern science. Plant polyphenols and natural products continue to be investigated for effective treatments for the most persistent of human ailments. In this review, fifty novel plant phenolic compounds have been compiled and briefly described from the previous five years. Select compounds and notable plant species from genus Morinda and Sophora are further expanded on. Traditional medicine plants often contain rich and diverse mixtures of flavonoids, from which rare compounds should receive attention. The bioactivity of crude plant extracts, purified compounds and mixtures can differ greatly, requiring that these interactions and mechanisms of action be investigated in greater detail. Novel applications of uncommon natural products, namely mimosine and juglone, are explored within this review. The 2019 coronavirus pandemic has resulted in abrupt spike of related scientific publications: speculation is made regarding plant natural products and future of antiviral drug discovery.
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Background: Antiviral vaccine is not effective, synthetic antiviral drugs are highly toxic, leading to increased interest in herbal medicines as promising antiviral drugs. Recently, Vipdervir has been developed from medicinal herbs with the aim to support and treat diseases caused by viruses such as H5N1 and SARSCoV- 2. In the present study, we assessed Vipdervir's antiviral activity against H5N1 and SARS-CoV-2. In addition, we also evaluated the acute toxicity and repeated dose toxicity of Vipdervir in mice and rabbits, respectively. Methods: H5N1 inhibitory effect of Vipdervir was assessed using hemagglutination inhibition assay. Vipdervir's SARS-CoV-2 inhibitory effect was evaluated by Plaque Reduction Neutralization assay. Acute and repeated dose oral toxicities of Vipdervir were determined according to OECD 423 and OECD 407 guidelines, respectively. Results: Data show that Vipdervir is effective against both H5N1 and SARSCoV- 2. At concentrations of 3 mg/mL and 5 mg/mL Vipdervir completely inhibits H5N1. At a concentration of 50 g/mL Vipdervir showed an inhibitory effect on SARS-CoV-2. Acute toxicity data revealed that the LD50 of Vipdervir is greater than 35200 mg/kg, b.wt. in mice. Repeated toxicity data indicated that Vipdervir did not induce significant differences in body weight gain, hematology and clinical biochemistry in compared to the control group. The No Observed Adverse Effect Level of Vipdervir is greater than 613.8 mg/kg b.wt./day in rabbits. No delayed toxicity effects of Vipdervir were observed. Conclusion: Vipdervir capsules were found to be antiviral effective and relatively safe in the tested doses and experimental conditions.
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The Soybean Science Challenge (SSC) continues to support Arkansas STEM (science, technology, engineering, and mathematics) educational goals. It aligns with the Next Generation Science Standards (NGSS). Junior high and high-school students are engaged in active learning and the co-creation of knowledge through the support of classroom-based lessons and applied student research. The SSC educates and engages junior high and high school science students and teachers in 'real-world' Arkansas-specific soybean science education through an original NGSS-aligned curriculum in 7E and GRC-3D format and a continuum of educational methods, which include: teacher workshops, online and virtual live stream education, virtual NGSS aligned mini-lessons for the science classroom, community gardens, personal mentoring, student-led research and corresponding award recognition, and partnerships with state and national educators, agencies and the popular media. The COVID19 global pandemic continued to alter the educational landscape in 2021, despite increased in-person instruction. The Soybean Science Challenge (SSC), by nature of its existing design and methodology, launched online Next Generation Science Standards (NGSS) aligned Gathering Reasoning and Communicating (GRC)-3D and 7E lesson plans for teachers. An online course was added, including NGSS-aligned mini-lesson videos for the science classroom, and additional virtual field trips were added to the list on the Soybean Science Challenge website. The Challenge also sponsored the Arkansas Science Teacher Association Conference in October 2021, and the SSC Coordinator Diedre Young conducted a workshop on bringing agriculturally based lessons into the science classroom. The Soybean Science Challenge was also active in science fairs across the state, judging participants at both the regional and state levels. The SSC is in its second year of the junior level award at regional science fairs. Through the SSC, teachers now have access to a plethora of educational instructions that bring real-world agricultural critical thinking into the classroom and students' homes. The SSC has learned that not only do Arkansas teachers and students benefit from these additional resources but teachers and students from other states benefit as well. In 2021, the SSC program reached over 3,000 students and teachers through in-person, digital, virtual, and print methods.
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ADMEN prediction was used to perform the first round screening from Traditional Chinese Medicine Database and Analysis Platform(TCMSP). then VASARA and molecular docking were used to screen again based on targets spike glyoprotein and angiotensin converting enzyme 2. and finally the interaction between target and drug was analyzed. 425 candidate ingredients of traditional Chinese medicine were screened from TCMSP database. when targeted by Spike glycoprotein. 12 ingredients were screened. They were contained artemisia apiacea salvia miltiorrhiza bge. scutellaria baicalensis. pinellia ternate. liquorice. radixImplettri and other traditional Chinese medicine. With ACE2 as the target. 77 components of traditional Chinese medicine were screened out. including salvia miltiorrhiza bge scutellaria baicalensis. pinellia ternatc. Liquorice. radix bupleuri. ephedra and other traditional Chinese medicine. At last. salviolone and dihydrotanshinlactone were found to be the potential inhibitor.
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COVID-19 is an infectious disease caused by a newly discovered CORONAVIRUS. It's a type of Severe Acute Respiratory Syndrome. The symptoms of SARS-nCOV-2 cause dry cough, fever, Tiredness, and difficulty of breathing (severe cases). We can cure the symptoms and defects of the whole body (vata, pitta, kapha) caused by the system and its qualitative therapists.
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In this study eco-friendly antimicrobial finish was developed by Azadirachata indica, Butea monosperma and Litchi chinensis and then applied on 100% silk fabric. The effectiveness of antimicrobial finish was checked up to 25 washes. The antimicrobial finish was extracted from leaves of these plants and applied on silk fabric by using pad dry cure method. It is true experimental study with pre-test and post-test control group design. FTIR and SEM tests were used to observe the presence of antimicrobial finish on silk fabric. The antimicrobial finish was fixed on silk fabric by the use of poly urethane binder. The antimicrobial activity of treated and untreated fabric and durability to successive washes were assessed by using ASTEM E2149 shake flask method. The treated fabric with A. indica and B.monosperme showed 100% reduction in microorganism presence while L. chinensis revealed 60% reduction against microorganisms. The antimicrobial finish showed 100% reduction after successive laundring up to 25 washes. This fabric is beneficial for home furnishing, sportswear and footwear industry as well as in apparel sector such as in formal wear and making matching face mask against COVID-19 pandemic.
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Low self-sufficiency rate and the higher foreign dependence rate of about 87% have seriously threatened the soybean safety in China. In recent years, with the changes of the international situation and the COVID-19 epidemic, soybean production, processing and consumption in China have attracted more attention at home and abroad. China unveiled "No. 1 central document" proposed a soybean revitalization plan in 2019, putting forward the goal of "one expansion and two improvements" to strengthen the competitiveness of China's soybean industry in the international agricultural market. This paper reviewed the current situation of soybean production, processing, trade and consumption in China in recent years, and upgraded strategies to enhance China's soybean industry and enhanced the self-sufficiency rate according to China's national conditions.
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Originally, the 17th Symposium on Insect-Plant Relationships (SIP-17) was scheduled to take place in Leiden, The Netherlands, in July 2020. However, due to the COVID-19 pandemic, the symposium was postponed to July 2021 and held in an exclusively online format. This exceptional edition has resulted in four strong contributions to the journal. It is with great pleasure that we now present a themed issue including the proceedings of SIP-17, supplemented with eight regular articles within the subject of insect-plant relationships.
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The current study is the reviews of the work done for pest infestation in different legume pulse grains Pulse grains have been found to have different susceptibility for the attack of pulse beetle. An insect has varied fecundity on different varieties of pulses. India is agriculture based country and agriculture is the key sector of Indian economy. Including many kharif and rabbi crops, pulses are cultivated in all parts of country. Pulse cultivation has its own significant place in agriculture as it has been considered as complimentary to cereal crop in many aspects and positioned as a second most important crop plant [1]. Study indicates 20-40% of dry matter of pulse grains is made up of beneficial dietary proteins [2]. Insufficiency of adequate amount of protein in diet may lead to protein energy malnutrition. Like poverty and hunger, to combat PEM is another great provocation for agriculture and nation. Pulse grains legumes are good source of protein and other nutrient materials. Crop plant of Arhar has its own advantage for agriculture. The leguminous plants are used in crop rotation to improve the fertility of soil as the root nodules harbor the nitrogen fixing bacteria;fixes atmospheric nitrogen to nitrogen compound about 72-350 kg/hectare/year . The current study emphasizes the different facts of pulse beetle and their infestation in arhar seed grains. About eight months of the year have been found to be susceptible for infestation;remarkable growth and development of pests continue during six months of the year. Arhar pulse grains are most suitable for pest infestation. Bold variety of arhar was identified to be the most preferred for maximum oviposition having larger and smooth surface area. Temperature suitable for oviposition was noticed between 28-370c. Developmental period was found to be of lesser duration and survival of adults was more in bold varieties as compared to the smaller variety. Developmental period was recorded as 24-30.3 days in bold legume grains and 26-32.3 days in smaller size grains. Survival of male and female insect pest was also different as male survived for 7-10.6 days and females for 8.3-12.6 days. Increased growth rate of insect population was there with the increase in temperature and humidity. Significant Weight loss, content loss and percent germination loss have been found in the pulse grains of both varieties in the current studies. Indian economy relies significantly on agriculture and loss and damage of complementary crop plant certainly affect it. At this point there is need to consider the condition of Mass poverty of India after pandemic covid -19 which has been ascertained after more than 45 years The outcome of pandemic recession is more than 2 fold increase in the number of poor from 60 million in a year to 134 million (Reports of downtoearth.org.in). Furthermore any rise in price would snatch the accessibility of the easily available protein diet from people living in indigence.
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The COVID-19 pandemic presented many challenges for the LSU AgCenter 4-H program. To meet social distancing guidelines and ensure the safety of youth and agents, many of programming efforts transitioned to a virtual format. However, the need remained for hands-on learning opportunities. 4-H programming is driven by the motto "learn by doing", and it was paramount that agents provide purposeful and engaging activities. The 4-H Seed-of-the-Month Club is a project club that emerged to meet that need. Agents created a nine-month seed subscription made available to youth across the region beginning in August 2020. With this project, participating youth were provided seeds of various plants, including pine seedlings, rainbow carrots, easter egg radishes, lettuce, green beans, okra, squash and coneflowers. Agents used the LSU AgCenter Louisiana planting guide to select the seeds to offer each month. In the future, agents and youth alike have expressed an interest in continuing to provide horticulture opportunities through the delivery mode of the Seed-of-the-Month Club. Participants were asked to provide top choices for their gardens by receiving carrots, lettuce and green bean seeds for the future.
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The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is causing coronavirus disease 2019 (COVID-19) pandemic. Ancient Chinese herbal formulas are effective for diseases caused by viral infection, and their effects on COVID-19 are currently being examined. To directly evaluate the role of Chinese herbs in inhibiting replication of SARS-CoV-2, we investigated how the phytochemicals from Chinese herbs interact with the viral RNA-dependent RNA polymerase (RdRP). Total 1025 compounds were screened, and then 181compounds were selected for molecular docking analysis. Four phytochemicals licorice glycoside E, diisooctyl phthalate, (-)-medicocarpin, and glycyroside showed good binding affinity with RdRp. The best complex licorice glycoside E/RdRp forms 3 hydrogen bonds, 4 hydrophobic interactions, 1 pair of Pi-cation/stacking, and 4 salt bridges. Furthermore, docking complexes licorice glycoside E/RdRp and diisooctyl phthalate/RdRp were optimized by molecular dynamics simulation to obtain the stable conformation. These studies indicate that they are promising as antivirals against SARS-CoV-2.
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Objective: To investigate the possible mechanism of Platycodonis Radix-Licorice drug pair in the intervention of COVID-19 by using network pharmacology and molecular docking technique. Methods The database TCMSP was retrieved for the chemical constituents and targets of Platycodonis Radix-Licorice drug pair. Coronavirus disease targets were screened by the Gene Cards, OMIM,TTD, PharmGkb and DrugBank database. Cytoscape 3.7.2 software was used to construct the drug-component-target network. The PPI(protein-protein interaction) network was obtained by drug-disease intersection targets, and the core genes were found through CytoNCA plug-in. Meanwhile, GO(gene ontology) analysis and KEGG(Kyoto encyclopedia of genes and genomes) pathway analysis were performed by using Bioconductor database to predict the mechanism. AutoDock Tools 1.5.6 software was used to simulate the molecular docking of the main active ingredients with the novel coronavirus key binding site protein [SARS-CoV-2 main protease(severe acute respiratory syndrome coronavirus 2 main protease, Mpro) and ACE2(angiotensin converting enzyme 2)]. Results A total of 7 active ingredients of Platycodonis Radix,92 active ingredients of Licorice,2766 drug targets, and 674 disease targets were obtained, and 67 drug-disease common targets were excavated. The key targets involved RELA,STAT1,MAPK3,TP53,MAPK1,MAPK8,STAT3,MAPK14,IL1 B and TNF by the database STRING and CytoNCA plug-in.Go enrichment analysis showed that the main functions of Platycodonis Radix-Licorice drug pair on the intervention of COVID-19 were antioxidant reaction, cell respond to chemical stress, regulation of apoptotic signaling pathways, reaction to lipopolysaccharides and reaction to bacteria-derived molecules, etc.. KEGG pathways involved Coronavirus disease-COVID-19 pathway, IL-17 signaling pathway and so on, were mainly associated with immune response, inflammation-related pathways, inhibition of viral infection, and other inhibition of cancer. The molecular docking results showed that glepidotin A,quercetin, licochalcone a and luteolin had good binding ability with Mpro and ACE2. Conclusion Platycodonis Radix-Licorice drug pair act on SARS-CoV-2 through multiple components, multiple targets, and multiple channel combination. And the main active ingredients have a fine binding ability with Mpro and ACE2. The method can provide theoretical support for the possibility of traditional Chinese medicine(TCM) against COVID-19.
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Objective: To explore the network regulation mechanism of Huoxiang Zhengqi Oral Liquid(HXZQ) in the treatment of coronavirus disease 19 (COVID-19).
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This issue contains 17 papers providing information on the newly discovered biological activities of phytochemicals and plant extracts that can be potentially developed as treatments for various disorders (COVID-19, cancer, skin disorders, allergies and inflammation, among others) and the underlying mechanisms by which they affect biological systems.
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The year 2020 marked one of the biggest recessions in global economic activity and world trade. During this period, the EU economy contracted by 6% and its international trade followed a similar downward trend - EU exports of goods decreased by 9% and imports by 12%, compared to 2019. By contrast, EU international trade in agri-food reported a slight growth. Over the course of 2020, the value of EU agri-food exports increased to 184.3 billion (a growth of 1.4% compared to 2019), while the value of imports rose to 122.2 billion (a growth of 0.5%). As a result, the EU further reinforced its leading position among the world's biggest exporters. On the import side, the EU has become the third largest importer after the US and China. The contraction in global trade was accompanied by increasing prices of food, including commodities as evidenced by the increases reflected in the FAO Global Price Index. The EU exports a wide range of products from all parts of the value chain which demonstrates the competitiveness of the EU agri-food sector in a variety of product classes ranging from commodities to highly processed food industry products. EU imports, on the other hand, are clearly dominated by basic agricultural food and feed products, which represent about 75% of all imports. Looking at product categories, exports of pig meat and wheat strongly contributed to the increase in EU overall agri-food exports. Conversely, spirits and liqueurs as well as wine are among the sectors that experienced a difficult period for a number of reasons (e.g. the COVID pandemic, US retaliatory tariffs). The growth in EU agri-food imports was mainly driven by increases in import values for oilseeds, other than soya beans;fatty acids and waxes, palm oil, fruit including tropical fruit, and soya beans. China, Switzerland and the Middle East and North Africa (MENA) region were the major growth destinations for EU agri-food exports in 2020. The value of EU exports fell most to the United States, Turkey, Singapore and Japan. In terms of imports, Canada grew significantly as a source for the EU imports. By contrast, EU imports declined most in value from the United Kingdom, Ukraine and the United States. In 2020, the UK has become the EU's most important partner in agri-food trade, with a share of 23% in total EU agri-food exports and 13% in total imports. With EU exports and imports both decreasing, its trade surplus with the US increased by 2% when compared to 2019, as falls on the imports side were stronger. China became the top destination for US agri-food exports. EU agri-food exports to China were primarily driven by continued record high sales of pig meat which increased by 74%. Pig meat and meat offal - the latter mainly comprised of products originating from pigs - accounted for over 40% of EU exports to China in 2020, demonstrating the importance of this market for the pig meat sector. Brazil's exports to China continued to increase in 2020, absorbing 35% of its total agri-food exports. Combined agri-food exports from Brazil to the EU and the US now account for half of Brazilian exports to China. In 2020, Brazil supplied 50% of extra-EU demand for soya beans and 40% for oilcakes. Wheat continued to be the leading EU export product to Africa with a 23% share of the EU's total export basket, whereas cocoa beans dominate in the EU imports from Africa, with the same share of 23%. Most African countries benefit from duty-free, quota-free access to the EU market under the "Everything But Arms" scheme and for many of them Economic Partnership Agreements (EPAs) or other trade agreements with the EU are applied, encouraging regional cooperation and trade. In 2020, the EU applied 45 free trade agreements (FTAs) with 77 partners. The share of agri-food trade under preferential agreements is also expanding and in 2021, it accounted for 31% and 41% of total EU agri-food exports and imports, respectively. The value of EU agri-food trade under preferential agreements expanded more in relative terms compared to total EU agri-food trade. EU agri-food ex